Image restoration and analysis of biomolecular radioactivity images

Biomolex has technology for real-time imaging of radioactive emissions. Images are acquired for measuring radioactivity in tissue slices for assessing transport and uptake of labeled biomolecules or in spots in protein kinase arrays for evaluating protein phosphorylations. The sensor used is a silicon strip detector, and a problem is that some strips produce no signal. Also, some other strips might have too high or low signals. Amethod for restoring the images and imputing themissing data has been developed for images of tissue slices. For the protein kinase arrays a method for estimating the radioactivity in each spot in the image has been developed. The image after missing data have been imputed, is one of the outputs when applying this method. The Biomolex technology opens up for the possibility of using multiple isotopes during a single image acquisition. A method for decomposing the measured signal into signals for the different isotopes is proposed and tested on simulated data.